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Methods published for rapid (≤ 60 days) in vitro generation of astrocytes from stem cell-derived NPCs.

Journal: PLOS ONE

Article Title: Efficient derivation of functional astrocytes from human induced pluripotent stem cells (hiPSCs)

doi: 10.1371/journal.pone.0313514

Figure Lengend Snippet: Methods published for rapid (≤ 60 days) in vitro generation of astrocytes from stem cell-derived NPCs.

Article Snippet: On Day 21, astrocyte progenitor cells were plated into Matrigel-coated plates (25.000–75.000/cm 2 ) in serum-free astrocyte maturation medium (AMM), which was AGM supplemented with 1x AGS, 1% Pen/Strep and 20 ng/mL recombinant human ciliary neurotrophic factor (CNTF, provided by Peprotech).

Techniques: In Vitro, Glutathione Assay